产品货号：
GS2224
中文名称：
Bmu I内切酶
英文名称：
Bmu I
产品规格：
250U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
Enzyme is active in presence of EDTA.
技术规格
相关搜索：Bmu I内切酶，Bmu I限制性内切酶
使用说明
Enzyme is active in presence of EDTA.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA(HindIII-digest)in 1hourat 37℃ in a total reaction volume of 50μl. |
| Recognition sites | ACTGGG(N)5↑ |
| 来源 | Bacillus megaterium S87 |
| Assayed on | Lambda DNA |
| Optimal buffer | Y(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.) |
| Optimal temperature | 37℃ |
| Storage conditions | 10 mM Tris-HCl(pH 7.5);250 mM NaCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;50%glycerol.Store at -20℃ |
| Ligations | After 2-fold overdigestion with enzyme about 75%of the DNA fragments can be ligated and 95%may be recut. |
| Non-specific hydrolisis | Overnight digest with BmuI is not recommended.A 50 μl reaction containing 1μgof Lambda DNA and 0.5 units of enzyme incubated for 4hoursresulted in the same pattern of DNA bands as a reaction incubated for 1 hour. |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 65℃ for 20 mins. |
| Campatible Ends | BfiI,BmrI |
相关搜索：Bmu I内切酶，Bmu I限制性内切酶